Coding
T18

Part:BBa_K1218015:Design

Designed by: Alex Constantino   Group: iGEM13_Stanford-Brown   (2013-08-29)


Bordetella pertussis adenylate cyclase subunit T18


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal AgeI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the standard Bacterial Adenylate Cyclase Two-Hybrid assay.


Source

PCR amplified from Bordetella pertussis crude cell lysate

References